Sperm damage in cold storage process is due to form ice crystals inside and outside the cell [17]. This phenomenon occurs during hypothermia and cold shock phenomenon called. Can limit the sperm lesions due to conditioning by methods such as improving the quality of semen before the cold preservation of sperm selection, to preserve, use the best preservative and applied the techniques of flax-proper defrost work [23].Verza s Jr (2009) study on normal semen sample 16 samples minimum essential is made more frozen cycles gave results: sperm are resistant to damage by cold preservation process and normal sperm samples to withstand the cooling process is much longer than the minimum sample average ejaculation 2 cycle. Resistance to the existing wounded by conditioning is related to the quality of the semen originally [66].The study "the influence of the ascorbatẹ and catalaza up the sperm in the cold preservation process" on 30 normal semen samples, Li z (2010) has proven: preserving the conditioning caused the injury to the person can sperm through the formation of oxidative radicals (R0S)
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