1.3.1 FLOCSTRUCTURE:CORRELATIVEMICR

1.3.1 FLOCSTRUCTURE:CORRELATIVEMICROSCOPY
Leppard
70
defined correlative microscopy (CM) as a strategy of using multiple
microscopic techniques which can include conventional optical microscopy (COM),
confocal laser scanning microscopy (CLSM), and transmission electron microscopy
(TEM), and allow one to detect, assess, and minimize artifacts that might arise from
using one technique only. CM has been successfully used by Liss et al.
61
with a min-imal perturbation approach in studying natural and engineered flocs. A recent minimal
perturbation approach
49,50
involves the use of sample stabilization in low melting
point agarose and a fourfold multi-preparatory technique. The use of a fourfold
multiple preparatory technique and CM has revealed how to maintain the structural
integrity of the samples through the stabilization, staining, and washing procedures.
The use of only one microscopic technique can bias or limit the information acquired
because of the artifacts that arise in specific sample preparations and the resolution
constraint associated with a particular technique.

Kết quả (Anh) 1:

FLOCSTRUCTURE 1.3.1: CORRELATIVEMICROSCOPYLeppard70defined correlative microscopy (CM) as a strategy of using multiplemicroscopic techniques which can include conventional optical microscopy (COM),confocal laser scanning microscopy (CLSM) and transmission electron microscopy(STAMPS), and allow one to detect, assess, and minimize artifacts that might arise fromusing one technique only. CM has been successfully used by Liss et al.61with a min-imal perturbation approach in studying natural and engineered flocs. A recent minimalperturbation approach49.50involves the use of sample stabilization in low meltingpoint agarose and a fourfold multi-preparatory technique. The use of a fourfoldmultiple preparatory technique and CM has revealed how to maintain the structuralintegrity of the samples through the stabilization, staining, and washing procedures.The use of only one microscopic technique can bias or limit the information acquiredbecause of the artifacts that arise in specific sample preparations and the resolutionconstraint associated with a particular technique.

Kết quả (Anh) 2:

1.3.1 FLOCSTRUCTURE: CORRELATIVEMICROSCOPY
Leppard
70
defined correlative microscopy (CM) as a strategy of using multiple
microscopic TECHNIQUES include conventional optical microscopy can mà (COM),
confocal laser scanning microscopy (CLSM), transmission electron microscopy and
(TEM), and allow one to detect, Assess, and minimize artifacts có Might Arise from
using only one technique. CM bị successfully used by Liss et al.
61
with a min-imal perturbation approach in studying natural and engineered flocs. A recent minimal
perturbation approach
49.50
dùng sample involves stabilization in low melting
point agarose and a fourfold multi-Preparatory technique. The use of a fourfold
multiple Preparatory technique and CM has revealed how to Maintain the structural
integrity of the samples through the stabilization, staining, washing and Procedures.
The use of only one microscopic technique can bias or limit the information acquired
the artifacts vì có Preparations Arise in specific sample and the resolution
constraint associated with một technique.