The subject is made through 3 experiment: (i) survey of the influence of the ratio of the solvent phase to separate the effect of the method, (ii) survey of the influence of the line speed to separate the effect of the method, (iii) determine the Vitamin A from different oil sources by HPLC system.The research results showed that can use the mobile phase solvent ratio ACN: H2O (70:30) and the speed of the line is 1 ml/min for high efficient method for washing time, relatively short award, the stable form, the broad peak doãng low and save solvent in the process of running the chromatograph.
đang được dịch, vui lòng đợi..