Lesson 5: biochemical reactions
A. Biochemical reactions with rabbit serum
Preparation: micropipette, 2 test tubes, Bunsen burner, implants, saline sterile, lyophilized rabbit plasma.
Purpose: Test the ability of plasma coagulation by enzymes coagulase.
Procedure:
- Use suction micropipette 1ml sterile saline solution into vials for lyophilized rabbit plasma. Then roll lightly in the hand to completely dissolve the plasma.
- Using a suction pipette earlier 1ml sterile saline into the tube for fossil NA tilt and shake until the turbid suspension forming.
- Using a micropipette smoking 0,5μl inoculum entered rabbit serum vial and then spread slightly in the palm.
- Then incubate and see results after 4/6/8/12.
results: after 12h the bottle slightly tilted rabbit plasma no coagulation phenomenon so conclusions bring test bacteria samples not Sta.aureus
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