Weight 1 g of soluble pepton using distilled water to 1000 ml, heated if necessary. Adjust the pH with NaOH 1 m or 1 m HCl solution to pH 7.0 achieved the sterilization after 0.2 at 25° c (if needed). Sterilization by autoclave sterilization at 121oC, 15 minutes in duration. Stored in a refrigerator at 2-8oC
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