o Use sterile pipet 1 ml of sample diluent for non-was in the middle of Petri. Pouring about 15 ml of the environment remain at 44-470 c VRBA on each disk Petri. Mix well with transplants translation by shaking under clockwise and vice versa. Let the mixture freezes again naturally by Petri in the horizontal plane, cool. At the same time, prepare a test disc with 15 ml to check the sterile
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