Current methodologies used to detec

Current methodologies used to detect porcine reproductive
and respiratory syndrome virus (PRRSV) infection include
1) virus isolation; (2) antibody detection, e.g., indirect immunofluorescence,
indirect immunoperoxidase monolayer
assay, enzyme-linked immunoasorbent assay, and virus neutralization;
3) antigen detection, e.g., immunohistochemistry
and immunofluorescence tests on tissue samples; 4) viral nucleic
acid detection, e.g., reverse transcriptase polymerase
chain reaction (RT-PCR) for detection and RT-PCR product
restriction fragment length polymorphism (RFLP) analysis
for strain typing.3 Primers from the open reading frame
(ORF) 7 (nucleocapsid gene) of PRRSV have been shown
to be specific for detection of all North American and European
isolates.2 In addition to detection of the virus, RTPCR
RFLP typing methods have been developed that make
it possible to differentiate different field strains and the commercial
vaccine

Kết quả (Anh) 1:

Current methodologies used to detect porcine reproductiveand respiratory syndrome virus (PRRSV) infection include1) virus isolation; (2) antibody detection, e.g., indirect immunofluorescence,indirect immunoperoxidase monolayerassay, enzyme-linked immunoasorbent assay, and virus neutralization;3) antigen detection, e.g., immunohistochemistryand immunofluorescence tests on tissue samples; 4) viral nucleic acidacid detection, e.g., reverse transcriptase polymerasechain reaction (RT-PCR) for the detection and RT-PCR productrestriction fragment length polymorphism (RFLP) analysisfor strain typing. 3 Primers from the open reading frame(ORF) 7 (nucleocapsid gene) of PRRSV STRAINS have been shownto be specific for detection of all North American and Europeanisolates. 2 In addition to detection of the virus, RTPCRRFLP typing methods have been developed that makeit possible to differentiate different field strains and the commercialvaccine

Kết quả (Anh) 2:

Current methodologies used to detect porcine reproductive
and Respiratory syndrome virus (PRRSV) infection include
1) virus isolation; (2) antibody detection, eg, indirect immunofluorescence,
indirect immunoperoxidase monolayer
assay, enzyme-linked immunoasorbent assay, and virus neutralization;
3) antigen detection, eg, immunohistochemistry
and immunofluorescence tests on tissue samples; 4) viral nucleic
acid detection, eg, reverse transcriptase polymerase
chain reaction (RT-PCR) for detection and RT-PCR product
restriction fragment length polymorphism (RFLP) analysis
for strain typing.

Kết quả (Anh) 3:

At present, methodologies is used to breed detect pigAnd respiratory syndrome virus infection (including PRRSV)1) virus isolation; (2) antibody indirect detection, for example, immunofluorescence,Indirect monolayer immunoperoxidaseAssay, enzyme linked assay immunoasorbent, and neutralization virus.3) antigen detection, for example, immunohistochemistryIn tissue samples and immunofluorescence test. 4) nucleic viralAcid detection, for example, transcriptase polymerase reactionChain reaction (today Russia - PCR) for the detection of PCR products and today RussiaLength analysis of polymorphism (RFLP) restriction fragmentStrain typing.3 from the open reading primers revolver(ORF) 7 (nucleocapsid gene) has been shown PRRSVSpecific to the large, is North America and Europe for all detectionIn addition, the study of large isolates.2 virus detection, RTPCRRFLP input method, so that has been developedIt is possible to distinguish between different live strains and big businessvaccine