Reactive oxygen species (ROS) react

Reactive oxygen species (ROS) react with cellular components, caus- ing oxidative damage to critical cellular biomolecules as lipids, proteins, and DNA. There is strong evidence that oxidative damage may play a significant role in the causation of several chronic diseases including ar - teriosclerosis, cataract, age-related macular degeneration, multiple scle- rosis, and cancer1,2.
Oxidative DNA damage may involve the breakage in single and dou- ble-strands, base modifications, fragmentation of deoxyribose, forma- tion of DNA-protein cross-links as well as abasic sites3,4. DNA strands breaks in eukaryotic cells can be detected by single cell gell electropho- resis (comet assay), with and without the addition of the repair enzymes endonuclease-III (Endo III), formamidopyrimidine N-glycosylase (Fpg), to characterize DNA lesions. Fpg initiates the repair of oxidized bases by excising them and cutting the sugar -phosphate backbone of DNA mol- ecule. Thus additional strand breaks are induced at the location of oxi- dized base, causing DNA relaxation and migration. The detection of Fpg- sensitive DNA lesions revealed the presence of oxidized purine bases5-7. Comet assay, which is a simple, sensitive, rapid and versatile assay, is commonly used for the assessment of protective effects of antioxidants on DNA damage in intervention studies8.

Kết quả (Anh) 1:

Reactive oxygen species (ROS) react with cellular components, caus there was-ing of oxidative damage to biomolecules as lipids, cellular critical proteins, and DNA. There is strong evidence that oxidative damage may play a significant role in the causation of several chronic diseases including ar-teriosclerosis, cataract, age-related macular degeneration, multiple scle rosis-, and cancer1 2.Oxidative DNA damage may involve the breakage in single and dou-ble-strands, base modifications, fragmentation of deoxyribose, forma-tion of the DNA-protein cross links as well as abasic sites3, 4. DNA strands breaks in eukaryotic cells can be detected by single cell electropho gell-resis (comet assay), with and without the addition of the repair endonuclease enzymes (Endo III), formamidopyrimidine glycosylase (Fpg)-N, to characterize the DNA lesions. Fpg initiates the repair of oxidized bases by excising them and cutting the sugar-phosphate backbone of DNA mol-ecule. Thus additional strand breaks are induced at the location of oxi-dized base, causing the DNA relaxation and migration. The detection of Fpg-sensitive DNA lesions revealed the presence of oxidized purines bases5-7. Comet assay, which is a simple, sensitive, rapid and versatile assay, is commonly used for the assessment of protective effects of antioxidants on DNA damage in studies8 intervention.

Kết quả (Anh) 2:

Reactive oxygen species (ROS) React with cellular components, caus- ing oxidative damage to cellular biomolecules critical as lipids, proteins, and DNA. Evidence There is strong sewing có oxidative damage play a role in the causation of the significant vài gồm Diseases chronic ar - teriosclerosis, cataract, age-related macular degeneration, multiple scle- rosis, and cancer1,2.
Oxidative DNA damage in sewing to involve the breakage single and dou- ble-strands, base modifications, fragmentation of deoxyribose, forma- tion of DNA-protein cross-links as well as abasic sites3,4. DNA in eukaryotic cells strands breaks can be detected by single cell resis electropho- gell (comet assay), with and without the Addition of the repair enzymes endonuclease-III (Endo III), N-glycosylase formamidopyrimidine (FPG), to characterize DNA lesions . FPG initiates the repair of oxidized bases by excising added and cutting the sugar backbone of DNA mol- ecule -phosphate. Strand breaks induced additional thì are oxi- dized at the location of the base, Causing DNA relaxation and migration. The sensitive detection of DNA lesions Fpg- revealed the presence of oxidized purine bases5-7. Comet assay, mà a simple, sensitive, rapid and versatile assay, is commonly used for the assessment of protective effects of antioxidants on DNA damage in studies8 intervention.