ABSTRACT The theme: "Research the characteristics of mushrooms Pestalotia sp. leaf spot disease caused mangosteen (Garcinia mangostana L.) "is performed in the laboratory of the Plant Protection Department, Faculty of Agronomy, Ho Chi Minh City University of Agriculture and Forestry. Leaf samples were collected in the garden of 12 provinces mangosteen: Ho Chi Minh City, Binh Duong, Binh Phuoc, Dong Nai, Vung Tau, Tien Giang, Tra Vinh, Vinh Long, Ben Tre, Gia Lai, Khanh Hoa and Hue. This study was conducted for the purpose: studying morphological characteristics and some biological features of mushrooms Pestaltoia sp., Examined the ability of spores to germinate Pestaltoia sp., Survey of fungal pathogenicity in the old leaves, potentially pathogenic fungi through zoning and by fungal spore suspension of Pestalotia sp. Through the survey process morphological characteristics of pathogens and disease symptoms on leaf of mangosteen may conclude spot disease Mangosteen is a fungal leaf Pestaltoia sp. cause. Spores germinate after 2-5 hours of incubation humidity, popular after 3 hours of incubation. Appropriate environment for the development of the OMA and CMA mycelium, a favorable environment for the formation of spores is PGA. Temperature 20 - 300C is suitable for the growth and sporulation of fungi, optimal at 250C, slow growing fungus and no sporulation at 150C and stop growing at 350C. appropriate pH from 5-7, especially at pH = 7, fungi Triem very slow and does not appear spores at pH 3. Results showed pathogenicity strains Pestalotia sp fungus. common disease in young leaves, leaf buds then and very little on the older leaves. Mushrooms do not cause disease in the absence of injury, in terms injure all isolates were showing symptoms. In laboratory strains of frozen mushrooms, symptoms are present after 2-5 days of challenge, common to 3 days after the race. Experiments with spore vaccine after 3-5 days showing symptoms, common in 4 days after race.
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