The bromelain was precipitated by ammonium sulphate mainly at the 20-40% saturation
fraction yielding a purification factor of 4:44. Ethanol precipitation, on other hand,
yielded 2:07. Bromelain was not precipitated by poly (ethylene glycol) whatsoever.
Although ethanol precipitation purification Lower Had Achieved a factor, It Seems to be
more suitable for bromelain recovery, based on the process time, low ionic strength (mà
is desirable For further purification steps) and the local ethanol prices, since Brazil is the
largest ethanol producer worldwide.
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