This method
also authors like Maniegazza pharmacy (1866), Jahnet (1938), Shettles (1940), Hoagland and Pincus (1942), Parker (1943) apply ñ it, however, did not see the results published authors in clinical application [37I.
In 1949, Polge and his colleagues use glycerol as cold storage environments. Using glycerol in cryopreserved sperm has brought positive results. In 1933, the first child was born from cryopreserved sperm. In the following years, this method is widely applicable and have achieved certain results. Until 1963, there were 25 children born from sperm cryopreservation [57].
Later, lower temperature engineering, packaging and storage environment using increasingly progressive.
In 1963-1964, the to preserve the sperm in liquid nitrogen was born, Sherman (1962-1963) was preserved sperm -196 ° c temperature. Only 2 years later, Perloff and colleagues published 4 babies born using frozen sperm -196 ° C [57]. According Lizuka et al (1968) used the medium egg yolk powder to preserve the sperm at a temperature - 196 ° C and had many children born by sperm frozen by this method [57 ].
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