II. Conducting experiments:1. Test on paper disc oxidase:-1 Small drops of sterile salt water up lame.-Use tongs to grab a paper disc Oxidase dipped in sterile saline drops for just enough damp (not wet paper disk do too) and put on lame.-Use sterile transplant ring retrieved khúm bacteria to disk wipe his paper. And read the results within 1 minute.2. Test on plastic table:-Use sterile swab taken for bacterial khúm in sterile salt water to make into legends that turbidity equivalent to Mc Farland 0.5 2.0.-Use a micropipette 200 µ l for legendary grab on the rigs on the plastic table. If you use the pipette Pasteur for the legends room at about 2/3 of wells.-Small parafilm for no. 1 wells and wells on the number 4. Each small wells 2 drops.-Plastic table cover and 35-37oC incubation/12-24 h.-Read the results after annealing for the experiments do not need drugs to try. Test reagents needed for small, then 1 drop of reagent respectively and read the results after adding reagents.3. environmental test on LDC:-Open the lid of the bottle and took 50 µ l micropipette (if using the Pasteur pipette, 1 drop) translation of myths (had to give in to the wells on a plastic Panel) to on and turn the bottle cap LDC environment. Note When for the right environment to translate into magical oils cone (or top of the pipette) through layers of parafilm and then pumped into the environment new LDC. Read the result after the 35-37oC incubation/12-24 h.4. Experiment on mobile environment:-Open the lid of the bottle and use a dental implants sterile needles obtained khúm bacteria and implanted into the vertical environment of MOB. Note plug the needle implanted making the bottom 1/3 of the bottle and withdraw the needle implanted vertically. Screw cap bottle.-Read the result after the 35-37oC incubation/12-24 h. results: bacteria not because red does not spread out of the implant.III. Results:Prototype information: Bacteria touch typical of e. coliThe result ID identifierYersinia aldovae 47Yersinia rohdei 21%Versinia mollareni 12%Versinia kristenseni 12%Versinia bercovieri 7%
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