The temperature was increased to 72 0 C makes the best DNA polymerase activity. Under the action of DNA polymerase and nucleotide primer in turn attached to the principle supplemented with mold circuit. The timing of this phase depends on the length of the amplified DNA sequences, usually lasts from 30 seconds to several minutes. The amplifier can be calculated as follows: Total amount of DNA amplification 2n = mx m: As the number of copies of the encrypted string. n: is the number of cycles. Thus, through a heat cycle, a target DNA was cloned into two copies; and if this cycle is repeated continuously 30 to 40 times, from a target DNA was cloned into a 230 to 240 copies, ie the hundreds of billions of stars.
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