After the samples were cultured in petri dishes we conduct environmental dump. PDA after being prepared and sterile environment we cooled down to about 40-45oC (this temperature is appropriate because if the temperature is too high will kill microorganisms and dangerous for people conducting experience, even if the temperature is lower, then the environment will be the east) and then poured into a petri dish was implanted around 15-20ml environmental samples so that the environment and government off the disk surface are rotating discs as 2D to disperse them sample the environment in order to create colonies left, then to the east to the environment (around 10-15p)
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